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1. What is the microflora? Where is it located in the digestive system? What is its composition? What is the role of the microflora in feed digestion?

2. What are the factors that upset the microflora and induce changes in the gut flora?

3. What is the basic concept of probiotics and how do they work?

4. Why are calves more susceptible to pathogens than adult cattle?

5. What are the minimum administration dose requirements for a probiotic?

6. What makes a good probiotic?

7. What exactly is Protexin?

8. What benefits are achieved by using Protexin?

9. When is the right time to use Protexin?

10. Are there any risks or disadvantages of using Protexin? Are there any risks of overdosing with Protexin?

11. How can the adult cow benefit from using Protexin?

12. How can the newly born calf benefit from using Protexin?

13. How can the calf at weaning benefit from Protexin?

14. Will Protexin fed animals be free of E. coli and Salmonella?

15. Can Protexin be used to prevent disease?

16. Why do probiotic trials sometimes show variable results?

17. Can Protexin be directly compared to Growth Promoters?

18. Is Protexin compatible with in-feed antibiotics, growth promoters or coccidiostats?

19. Is Protexin compatible with chlorine, water sanitisers and any other in-feed components?

20. How do Probiotics breakdown toxins and reduce the detrimental effects of mycotoxins in feed?

21. Does Protexin require any special storage methods?

What is the microflora? Where is it located in the digestive system? What is its composition? What is the role of the microflora in feed digestion?

The microflora

Calves at birth are born with a relatively sterile gut. Thus the newly born calf will be more easily colonised by pathogenic microorganisms as there is no protective microflora to reduce the colonisation of pathogens at this stage of life and the immediate environment to which the calf is born is contaminated with beneficial as well as potentially pathogenic microorganisms.

Soon after birth the calf begins to acquire a complex collection of microorganisms which populate its intestinal tract. This collection of microorganisms is termed as the microflora. The gut microflora contains a variety of different bacteria and fungi of which there are typically ~400 different types of microorganisms with a total population of ~ 1014 throughout the length of the intestinal tract.

Location in the digestive system

This complex collection of gut microorganisms is distributed throughout the whole length of the gut.  Within particular regions the organisms may be found in three niches:

(a) associated with gut wall. This can either take the form of direct attachment to the gut epithelium or entrapment in the mucous layer of the epithelium

(b) attachment to food particles

(c) suspension in the liquid phase of the gut contents

Composition

The composition of the flora varies in different regions of the intestine and is dependent on factors such as pH. The microflora which develops in the calf's intestinal tract is characteristic for that species, and is a very stable population which has evolved a symbiotic association with the host.

Role of gut flora in digestion

The microflora forms a symbiotic relationship with the host and benefits the host by aiding digestion by:

Producing various enzymes which are involved in the digestion/breakdown of large feed particles/polysaccharides such as cellulose.

The bacteria in the gut can also stimulate an immune response. For example, germfree animals have lower levels of gamma-globulin than do conventional animals with a complete gut flora. 
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